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Fluorescence Microscopy Digital Image Gallery

Rat Duodenum Tissue Sections

The small intestine is comprised of three major regions: the duodenum, jejunum, and ileum. The region closest to the stomach is the duodenum, which is separated from that structure by the pyloric sphincter. Once matter is passed from the stomach to the duodenum, it is exposed to bile and digestive juices. These substances, which aid in food digestion, are released into duodenum via the hepatopancreatic duct. Movement of the bolus through the duodenum and other parts of the small intestine is driven by peristalsis, wavelike muscular contractions.

Rat Duodenum Tissue Sections

The layered structure of the duodenum is similar to that of the stomach. Both feature an inner lining known as the mucosa, a supportive layer termed the submucosa, a muscular stratum, and an outer covering, or serosa. Brunner's glands, which secrete highly alkaline mucus, are located in the submucosa of the duodenum. This mucus modifies the pH of the chyme that passes into the duodenum, changing it from acidic to basic, and facilitates additional food breakdown. Specialized ducts enable the secretions of the Brunnerís glands to enter the lumen of the duodenum.

In order to localize a red fluorescent tag to filamentous actin in the sample of rat duodenum tissue presented in the digital image above, the specimen was labeled with Alexa Fluor 568 conjugated to phalloidin, a phallotoxin derived from the toxic death cap mushroom. Oregon Green 488 conjugated to the lectin wheat germ agglutinin, which selectively binds to N-acetylglucosamine and N-acetylneuraminic residues, was also applied to the tissue sample, as was the DNA probe Hoechst 33342. Images were recorded in grayscale with a 12-bit digital camera coupled to a Nikon Eclipse 80i microscope equipped with bandpass emission fluorescence filter optical blocks. During the processing stage, individual image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles.

Additional Widefield Fluorescence Images of Rat Duodenum Tissue Sections

Targeting Lectins and The Filamentous Actin Network in Rat Duodenum Tissue Sections - A number of disorders can affect the duodenum, as well as other parts of the gastrointestinal tract. Such disorders range from relatively minor problems, such as indigestion, to potentially very serious maladies, such as ulcers and cancer. Symptoms of duodenal disorders vary, but they often manifest themselves as bloating, abdominal pain, poor appetite, heartburn, nausea, or vomiting.


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