Fluorescence Microscopy Digital Image Gallery

Rat Duodenum Tissue Sections

Rat Duodenum Tissue Sections

A number of disorders can affect the duodenum, as well as other parts of the gastrointestinal tract. Such disorders range from relatively minor problems, such as indigestion, to potentially very serious maladies, such as ulcers and cancer. Symptoms of duodenal disorders vary, but they often manifest themselves as bloating, abdominal pain, poor appetite, heartburn, nausea, or vomiting. A few medical conditions may also become evident from the appearance of blood in the stool. Endoscopy is typically necessary for a diagnosis of duodenal or other gastrointestinal problems.

The rat duodenum tissue section illustrated in the digital image above was labeled with wheat germ agglutinin (WGA) conjugated to Oregon Green 488. WGA, which selectively binds to N-acetylglucosamine and N-acetylneuraminic (sialic acid) residues, is well suited for staining the Golgi network in fixed cells and tissues since a number of proteins and lipids found in the Golgi apparatus are glycosylated. The specimen was also labeled with Alexa Fluor 568 conjugated to phalloidin (targeting filamentous actin) and Hoechst 33342 (targeting DNA in the nucleus). Images were recorded in grayscale with a 12-bit digital camera coupled to a Nikon Eclipse 80i microscope equipped with bandpass emission fluorescence filter optical blocks. During the processing stage, individual image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles.

View a larger image of this rat duodenum tissue section.